الصف : الثاني عشر - العام الدراسي : 2016/2017


• Genome engineering is just about its dawn of golden age. New and exciting tools are arising and piling up to its arsenal.



• .After TALENs, Zing-finger a new tool is coming to add up, the newest addition is the CRISPR system, a system that firstly discovered at the Streptococcus thermophilus and its natural role is the sequence-specific adaptive immunity against foreign DNA in bacteria.



• In the present report, CRISPR Cas9, type II system, used in an attempt to knock out the HPRT1 gene expressed in lymphocytes as a proof of concept. HAT medium in combination with Thioguanine used as a selection medium enabling a quick and simple read-out screening method, to verify that a successful editing event took place in the mammalian cells, a detection DNA cleavage kit used in combination with an enzymatic assay.


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  • حلقة بحث

    GENETIC ENGINEERING Crispr-cas9

الإعداد :


  • حسن عمار

الإشراف :


  • نضال حسن

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