الصف :الثاني عشر - العام الدراسي :2016/2017


In the last two decades, the biological domain start growing rapidly for the humans good, the researchers start thinking in ways to know more and more about the human body, and they have succeeded in revealing a lot of secrets in the body, but one of the most important things that been discovered is western blotting, which is a procedure or an analysis to figure out the proteins that exist in the cell and their concentrations, you might say what is the use of knowing this things??, I will say: everything… it helps us to know what is happening in our bodies and what is wrong with them, like what are the diseases we have, what are the things that are not working correctly, and a lot of other important information.

We will talk in this research about the procedure of western blotting, from sample preparation until imaging the results, and we will mention some applications for it.

And in the end of this research you will be able to answer these questions:

  • How we know what proteins exist in our cells???
  • How we make this procedure??
  • What is the applications of this procedure??
  • Is there a certain way to do it, or it varies??? 

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    WESTREN BLOTTING

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